Spectrophotometric Determination of Carboxyhemoglobin
نویسندگان
چکیده
منابع مشابه
The spectrophotometric measurement of carboxyhemoglobin.
A simple, reliable and rapid method for the spectrophotometric determina tion of carboxyhemoglobin in fresh blood is described. A blood hemolysate is prepared by diluting a blood sample with 0.4 percent NH4 OH. Addition of sodium dithionite deoxygenates hemoglobin (and reduces methemoglobin if present) without affecting carboxyhemoglobin. Comparison of the 541/555 nm absorbance ratio for the h...
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This spectrophotometric method for estimating carboxyhemoglobin is based on the production of a two-pigment mixture by reducing oxyhemoglobin and methemoglobin with sodium hydrosulfite . Absorbances of the pigment are measured at 420 and 432 nm. Protecting the solutions against exposure to air is unnecessary. Values are stable and reproducible when the solutions are buffered at pH 6.85.
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This paper describes separate spectrophotometric procedures for rapidly measuring carboxyhemoglobin (COHb) and methemoglobin (MetHb) in blood. Absorbance measurements are made in the Soret region at a blood dilution near 1000-fold. For COHb estimation, the diluent contains sodium hydrosulfite, providing the two-component system COHb--Hb for absorbance measurements at 420 and 432 nm. The NA2S2O4...
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In this procedure hemoglobin is converted to its reduced form and the magnitude of the zero-order spectral shift of the reduced hemoglobin peak at 430 nm to the carboxyhemoglobin peak at 418 nm is determined by second-derivative spectrum analysis. The method is simple, straightforward to set up, and rapid. A result may be obtained within 15 min of receiving the sample. It is sufficiently sensit...
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ژورنال
عنوان ژورنال: Eisei kagaku
سال: 1969
ISSN: 0013-273X
DOI: 10.1248/jhs1956.15.342